Sperm Concentration Test

According to "WHO human semen laboratory manual" (5th edition), the main factors influencing the male sterility are: semen volume and total number of sperm, sperm concen- tration, liquefaction time, semen PH, normal morphology sperm rate, etc., including sperm concentration detection is an important indicator of male infertility. In this manual, the normal minimum sperm concentration is 15 x10⁶/ml, when sperm concentrations less than 15 x10⁶ /ml, that have fertili-ty problems. But the male sterility in combination with other indicators is needed to make a final judgment. Based on a number of anatomy, genetics and biochemical characteris tics, SP10 proteins were confirmed can be chosen for the analysis of sperm concentration, its only expressed in testis, and sperm production process is the last step of differentiation markers. SP10, according to the data showed that protein concentration and sperm concentra- tions show a certain linear relationship, namely when the sperm concentration of 15 or more x106/ml, SP10 protein detection were positive. So this reagent can be used in the auxiliary diagnosis of male infertility, at the same time provide fertility guidance for age-appropriate couples.